Double stranded DNA sequencing as a choice for DNA sequencing
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منابع مشابه
Double stranded DNA sequencing as a choice for DNA sequencing.
Double stranded DNA sequencing (1) is favored because of its simplicity, and convenice. However it is only recently that the quality of this method has become comparable with single stranded DNA sequencing. We believe that two factors have limited the popularity of double stranded DNA sequencing: 1, the quality of the template DNA; 2, the inherent propertv.,of the DNA polymerase. The modified T...
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Duplex probes with five base single-stranded overhangs can capture dsDNA targets from type IIS restriction nuclease digests. Ligation generates a predesigned nick site, where DNA polymerase can generate sequencing ladders by strand displacement or nick translation in the presence of trace amounts of dideoxynucleotides. This allows dsDNA targets to be captured from mixtures and directly sequence...
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The dideoxy sequencing technique has been applied to the d i rec t sequencing of large double-stranded DNA molecu les with a small single-stranded primer. For ins tance , the method was applied to the lambda genome, which contains 48 502 base-pairs (Sanger F, Coulson AR, Hong GF, Hill D & Petersen GB, 1982, J. Mol. Biol., in press), and the coding region for gene W identified. The procedure pro...
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Isolation of cell free fetal DNA (cffDNA) from maternal serum usually leads to very low concentrations of DNA impeding further resolving through conventional methods of electrophoresis. Although several protocols have been described for capillary electrophoresis (CE) of double stranded DNA, they usually need using special polymers or coated capillaries which degrade over time. Herein, we propos...
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branes by transillumination. Appl. Theor. Electrophor. 1:59-60. 5.Smejkal, G.B. and H.F. Hoff. 1993. Co-localization of molecular mass marker proteins on Western blots. BioTechniques 15:796-798. 6.Smejkal, G.B. and H.F. Hoff. 1994. Filipin staining of lipoproteins in polyacrylamide gels: sensitivity and photobleaching of the fluorophore and its use in a double staining method. Electrophoresis 1...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1988
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/16.3.1220